Multiphoton Imaging System

Cell Images using Multiphoton Imaging System
Cell Images using Multiphoton Imaging System
Multiphoton System
Multiphoton System
Multiphoton System
Multiphoton System
Multiphoton System
Multiphoton System

Multiphoton imaging creates possibilities for visualising fine structures in deep tissue and provides optical sectioning at high resolution. By scanning over a wide area it is possible to collect data simultaneously from up to several hundred cells.
Often viewed as an exclusive technique requiring significant funding and support expertise, Scientifica’s award-winning system overcomes these constraints and is ideal for electrophysiology integration.

Scientifica's multiphoton system offers both, Galvo Scanning and Resonant Scanning.

The Galvo Scanhead uses two galvanometer scan mirrors (one for each axis) to direct the laser beam to any xy position in the field of view.
Galvanometers offer the benefit of variable scanning speeds, adjustable to suit each individual application.
These scan mirrors facilitate frame rates of up to 15 frames per second for freely rotatable fields of view or 30 frames per second with bi-directional scanning. Highly flexible scan patterns such as arbitrary line scans, spiral scans or patterned point scanning can also be achieved.

The multiphoton system can also be fitted with a Resonant Scanner to acquire faster frame rates over the entire field of view.
This option is tailored to the large back aperture objectives so that 30 frames per second can be achieved at a resolution of 512 x 512 pixels. This enables researchers to monitor activity in a large population of cells.
Resonant scanning systems are a popular choice for two-photon calcium imaging using genetically encoded indicators (e.g. GCaMP) or other functional dyes (e.g. OGB-1, Fura-2).

Please see Scientifica's Galvo vs Resonant scanning guide for Pros and Cons of either method.

"We have been using two multiphoton imaging systems from Scientifica, and have been very pleased with their performance. The systems are robustly constructed and designed to be easily integrated with electrophysiology. We have also been very pleased with the after-sales support provided by Scientifica." - Professor Leon Lagnado, University of Sussex

Applications:

  • High resolution morphological imaging
  • Network studies
  • Three dimensional imaging
  • Calcium imaging
  • Embryo development studies
  • Microvasculature imaging

Please visit Scientifica's homepage for complete information

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